Genome Science

A Practical and Conceptual Introduction to Molecular Genetic Analysis in Eukaryotes

By David Micklos, Cold Spring Harbor Laboratory; Bruce Nash, Cold Spring Harbor Laboratory; Uwe Hilgert, University of Arizona

Genome Science is a textbook and laboratory manual for advanced secondary and post-secondary education. It combines approachable narrative with extensively tested lab exercises that illustrate key concepts of genome biology in humans, invertebrates, and plants. Nineteen labs, organized into four chapters, engage students with both bioinformatics exercises and in vitro experiments. Each chapter also includes an extensive introduction that provides an historical and conceptual framework. This modular structure offers many options for enhancing existing courses, starting new courses, or supporting student research projects. The book is complete with advice for instructors, laboratory planning guidelines, recipes for solutions, and answers to student questions.

Contents

  1. Genome as Information
    • Introduction
    • Lab 1.1 Annotating Genomic DNA
    • Lab 1.2 Detecting a Lost Chromosome
    • Lab 1.3 Comparing Diversity in Eukaryotes
    • Lab 1.4 Determining the Transposon Content in Grasses
    • Lab 1.5 Identifying GAI Gene Family Members in Plants
    • Lab 1.6 Discovering Evidence for Pseudogene Function
    • Laboratory Planning and Preparation
    • Answers to Questions
  2. The Human Genome
    • Introduction
    • Lab 2.1 Using Mitochondrial DNA Polymorphisms in Evolutionary Biology
    • Lab 2.2 Using an Alu Insertion Polymorphism to Study Human Populations
    • Lab 2.3 Using a Single-Nucleotide Polymorphism to Predict Bitter-Taste Ability
    • Laboratory Planning and Preparation
    • Recipes for Reagents and Stock Solutions
    • Answers to Questions
  3. Plant Genomes
    • Introduction
    • Lab 3.1 Detecting a Transposon in Corn
    • Lab 3.2 Detecting a Transposon in Arabidopsis
    • Lab 3.3 Linkage Mapping a Mutation
    • Lab 3.4 Detecting Genetically Modified Foods by Polymerase Chain Reaction
    • Lab 3.5 Using DNA Barcodes to Identify and Classify Living Things
    • Lab 3.6 Detecting Epigenetic DNA Methylation in Arabidopsis
    • Laboratory Planning and Preparation
    • Recipes for Reagents and Stock Solutions
    • Answers to Questions
  4. Genome Function
    • Introduction
    • Lab 4.1 Culturing and Observing C. elegans
    • Lab 4.2 Using E. coli Feeding Strains to Induce RNAi and Knock Down Genes
    • Lab 4.3 Examining the RNAi Mechanism
    • Lab 4.4 Constructing an RNAi Feeding Vector
    • Laboratory Planning and Preparation
    • Recipes for Reagents and Stock Solutions
    • Answers to Questions
  • Cautions Appendix
  • Equipment Appendix
  • Subject Index

 

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