Interviewee: Paul Berg.
Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA.
(DNAi Location: Manipulation > Techniques > Cutting & Pasting > Interviews > On the phenomenon of restriction)
Transcript:
Boyer, Herb Boyer had been studying restriction, the phenomenon of restriction. And he, along with a few others, realized and discovered that restriction, restriction was defined as if you took DNA from one organism and put it into another they destroyed it. But if you took their own DNA and put it in, it went in and survived, so there, there had to be some way in which a given organism recognized its own DNA and also recognized somebody else's DNA as being foreign and destroyed it.
Keywords:
herbert boyer,manipulation techniques,recombinant dna,paul berg,dnai,interviewee,bacterium,restriction,organism,phenomenon
Paul Berg's student, Janet Mertz, planned an experiment that would recombine DNA from a monkey virus with DNA from a bacterium that lives in the human gut. Berg describes colleague Bob Pollack's outrage at this.
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.