Website Search
ID 15065
Switching genes off?, Mario Capecchi
Description:
Interviewee: Mario Capecchi.
Switching genes off?
Transcript:
There are techniques for being able to put a gene under a switch, so one way is to simply turn it off completely for all time with respect to the embryo, and that tells you all the places the gene is, is doing something. And unfortunately it may even kill the embryo and then you won't even be able to study, for example, what is this gene doing in the adult, okay. So to obviate that problem, what we do is to put it under a switch, and then we'll be able to turn it off in a particular space, for example the limb bud, or in the brain, and also at different times. We can look at it at different stages of embryogenesis and in fact we can wait and allow the gene to operate and make an adult, and then turn it off and ask what is this gene doing in the adult.
Keywords:
limb bud,interviewee,different stages,different times,embryo,genes,mario,brain,adult
Downloads:
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 15062. Technique to control a gene (Part 2), Mario Capecchi
- Mario Capecchi continues his explanation of the technique he uses to control genes in mice.
- SOURCE: DNAi
- 15060. Homologous recombination, Mario Capecchi
- Mario Capecchi discusses homologous recombination, the technique he developed to introduce a desired mutation into the DNA of living cells.
- SOURCE: DNAi
- 15064. Mouse models for limb formation, Mario Capecchi
- Mario Capecchi discusses the similarities between mouse and human limb formation, and how his work might lead to limb regeneration in the future.
- SOURCE: DNAi
- 15066. Advantages of mouse models, Mario Capecchi
- Mouse researcher Mario Capecchi talks about the similarities in mammalian anatomy and physiology.
- SOURCE: DNAi
- 15070. An artificial chromosome?, Mario Capecchi
- Mario Capecchi talks about the possibility of introducing an artificial chromosome into cells to slow the aging process.
- SOURCE: DNAi
- 15059. Gene targeting, Mario Capecchi
- Mario Capecchi talks about manipulating embryonic stem (ES) cells to make specific mutations in mouse embryos.
- SOURCE: DNAi
- 15965. What is Prader-Willi/Angelman?
- Genes on chromosome 15 show an effect called "imprinting." The effect of mutations in these genes varies depending on whether they are inherited from the mother or the father, which appears to play different roles in human development. Genes from the fath
- SOURCE: DNAi
- 16856. Animation 41: DNA is only the beginning for understanding the human genome.
- Mario Capecchi describes proteomics; the large-scale study of protein structure and function. Brian Sauer explains gene knock outs.
- SOURCE: DNAFTB
- 15042. The first day of clinical trials for Gleevec, Bud and Yvonne
- Yvonne, Bud's wife, talks about the first day of the clinical trials in June of 1998.
- SOURCE: DNAi
- 16760. Animation 37: Master genes control basic body plans.
- Eric Wieschaus and Christiane Nüsslein-Volhard explain research of Drosophila's developmental stages, and Ed Lewis presents homeotic mutations.
- SOURCE: DNAFTB
