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ID 15083

Making DNA chips, Stephen Fodor

Description:
Interviewee: Stephen Fodor. Stephen Fodor talks about the photolithographic technique used to synthesize pieces of DNA on the surface of GeneChips™. (DNAi Location: Manipulation > Techniques > Large-scale analysis > Interviews > GeneChips™, step by step)
Transcript:
The way we make these DNA chips is that we actually build the DNA step by step at each location on one of these chips. In a process known as photolithography we excite with light a certain region on the chip and that prepares that particular site for chemical reaction. One of the four building blocks, A, C, G or T are applied and wherever we shine the light, that building block will stick and grow. And so we go through a multiple number of steps in this way, over literally millions of different sites on a large piece of glass, and what we get are literally millions of different precise locations on the chip, each with their own different strand of DNA on it.
Keywords:
stephen fodor,dna chips,manipulation techniques,dnai,precise locations,piece of glass,interviewee,chemical reaction,building blocks,strand
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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