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ID 15139

Finding DNA to copy, Kary Mullis

Description:
Interviewee: Kary Mullis. Kary Mullis speaks about the process of find a specific fragment of DNA amongst many pieces in a complex mixture. (DNAi Location: Manipulation > Techniques > Amplifying > Finding the DNA to copy)
Transcript:
When you're working with DNA, you're usually, you're only just interested in one little part of it at a time. You can't, you're not interested in the whole three billion base pairs. You might be interested in it but you can't deal with that many at once. So this was a way to focus, to pull the needle that you were interested in, out of this haystack full of all these other needles that looked really similar, to pull the one you wanted out and get as much of it as you wanted to work with. And it just, it changed the rules, molecular biology had always been limited by the problem of not being able to tell the difference between one sequence and another, nor to get it away from all the rest of 'em. They all look pretty much alike in a tube, you can't tell.
Keywords:
polymerase chain reaction,kary mullis,dna amplification,manipulation techniques,base pairs,dnai,molecular biology,interviewee,haystack,chain reaction,fragment,needles,mixture
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