Website Search
ID 15356

Determining the nucleotide sequences of all RNA codons, Marshall Nirenberg

Description:
Interviewee: Marshall Nirenberg. Marshall Nirenberg talks about Gobind Khorana, who synthesized many of the triplets needed to finish the decoding process. (DNAi Location: Code > Reading the code > Players > Har Gobind Khorana >Finishing the code)
Transcript:
Gobind Khorana is, was at that time the, one of the world's best organic chemists working in the field of nucleic acids, highly experienced and superbly equipped to do this. He synthesized the 64 triplets chemically and he also synthesized repeating polymers with known doublets, repeating doublets or triplets as well, and towards the end he used them all to determine nucleotide sequences also. So between the two labs, he used the ribosome binding assay that we had established and between the two labs we determined all of the nucleotide sequences of RNA codons.
Keywords:
rna codons,har gobind khorana,nucleotide sequences,organic chemists,marshall nirenberg,nucleic acids,dnai,amino acids,cracking the code,location code,interviewee,triplets,polymers
Downloads:
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Related content:

16512. Biography 22: Har Gobind Khorana (1922 - )
Har Gobind Khorana, Marshall Nirenberg, and Robert Holley shared the 1968 Nobel Prize for Physiology and Medicine. Nirenberg and Khorana cracked the genetic code. Holley sequenced and deduced the structure of the first tRNA molecule.
16511. Biography 22: Marshall Warren Nirenberg (1927- )
Marshall Nirenberg, Har Gobind Khorana, and Robert Holley shared the 1968 Nobel Prize for Physiology and Medicine. Nirenberg and Khorana cracked the genetic code. Holley sequenced and deduced the structure of the first tRNA molecule.
15353. Figuring out the other codons, Marshall Nirenberg
After decoding the "easy" codons, Marshall Nirenberg talks about his strategy for decoding the rest.
15350. The RNA code for phenylalanine, Marshall Nirenberg
Marshall Nirenberg talks about the RNA code for phenylalanine.
16494. Animation 22: DNA words are three letters long.
Several researchers crack the genetic code.
15883. The other codons: Nirenberg and the genetic code
After the easy codons, exact triplets had to be made in order to finish deciphering the rest. Marshall Nirenberg and a group of scientists including Maxine Singer, Marianne Grunberg-Manago, Phil Leder were involved in this process. Har Gobind Khorana al
15355. Making triplet codons, Marshall Nirenberg
Marshall Nirenberg talks about the contributions of Maxine Singer, Marianne Grunberg-Monago and Phil Leder.
15354. Origin of the RNA code, Marshall Nirenberg
Marshall Nirenberg talks about origin of the RNA code.
15352. Proving it was phenylalanine, Marshall Nirenberg
Having made phenylalanine using poly-U mRNA, Marshall Nirenberg still had to prove it. He talks about how he did this with help from a fellow scientist – Michael Siler.
15882. Breaking the code
Marshall Nirenberg and Heinrich Matthaei used poly-U mRNA in a cell-free system to make a polyphenylalanine protein chain. This showed that UUU must be the code that specifies the amino acid phenylalanine.
Cold Spring Harbor Laboratory
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving