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ID 15421

Cutting DNA with restriction enzymes, James Watson

Description:
Interviewee: James Watson. James Watson discusses the breakthrough that allowed scientists to cut DNA. (DNAi Location: Manipulation > Revolution > Players > James Watson > The big breakthrough)
Transcript:
Big breakthrough was finding ways to cut DNA molecules at specific spots, not randomly sheared, we'd always studied DNA which had been broken randomly, you had to break it at specific points, that was the real problem, how to cut at defined spots along the DNA molecule. And we thought all molecules which cut DNA, the degradative enzymes, cut randomly. So we didn't know how to proceed. And then suddenly, or over a couple of years, it began to appear there was a class of enzymes that cut DNA molecules at quite specific points.
Keywords:
restriction enzymes,dna molecules,dna molecule,james watson,recombinant dna,degradative enzymes,dnai,interviewee,manipulation,breakthrough,scientists
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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