Once scientists could cut DNA, they still needed a way to paste DNA strands together at will. Arthur Kornberg's identification of an enzyme he called ligase allowed scientists to paste the ends of DNA molecules together.
(DNAi Location: Manipulation > Revolution > pieces of the puzzle > DNA ligation)
Transcript:
This animation shows the alignment of matching sticky ends (in pink and red) and the remaking of the sugar-phosphate bonds that form the backbone of DNA by DNA ligase (in green).
Keywords:
dna ligation,restriction enzymes,dna ligase,restriction digest,backbone of dna,sticky ends,pieces of the puzzle,3d animation,dnai,phosphate,alignment,manipulation,bonds