Once scientists could cut DNA, they still needed a way to paste DNA strands together at will. Arthur Kornberg's identification of an enzyme he called ligase allowed scientists to paste the ends of DNA molecules together.
(DNAi Location: Manipulation > Revolution > pieces of the puzzle > DNA ligation)
This animation shows the alignment of matching sticky ends (in pink and red) and the remaking of the sugar-phosphate bonds that form the backbone of DNA by DNA ligase (in green).
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