Vats used to grow bacteria

How was synthetic insulin first made? Although the idea was simple, in practice there were substantial problems. No one had the human DNA sequence for insulin, and there was a moratorium on the production of human recombinant DNA. Two different strategies were used to obtain the human insulin DNA sequence, and transfer it into bacteria. In the end, one strategy and one company won out.

Vats used to grow bacteria.

How was synthetic insulin first made? Although the idea was simple, in practice there were substantial problems. No one had the human DNA sequence for insulin, and there was a moratorium on the production of human recombinant DNA.

Two different strategies were used to obtain the human insulin DNA sequence, and transfer it into bacteria. In the end, one strategy and one company won out.

human dna sequence,human insulin,recombinant dna,substantial problems,moratorium,bacteria

  • ID: 15644
  • Source: DNALC.DNAi

Related Content

15505. Synthesizing human insulin using recombinant DNA, 3D animation with no audio

Synthesizing human insulin using recombinant DNA, 3D animation with no audio

  • ID: 15505
  • Source: DNAi

15643. Genentech

Genentech, the first biotechnology company, established in 1976.

  • ID: 15643
  • Source: DNAi

15928. How insulin is made using bacteria

Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels. Synthetic insulin i

  • ID: 15928
  • Source: DNAi

15255. Producing human insulin using recombinant DNA, Walter Gilbert

Walter Gilbert talks about the reasons for making insulin with recombinant DNA.

  • ID: 15255
  • Source: DNAi

15258. Government restrictions on working with recombinant DNA, Walter Gilbert

In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.

  • ID: 15258
  • Source: DNAi

15259. Cloning the human insulin gene, Walter Gilbert

The recombinant DNA moratorium meant Gilbert had to go to England's Porton Down facility to try and isolate human insulin. He only had one chance ...

  • ID: 15259
  • Source: DNAi

15929. How insulin is made using yeast

Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels.

  • ID: 15929
  • Source: DNALC.DNAi

15927. Isolating DNA to make human insulin

Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.

  • ID: 15927
  • Source: DNAi

15256. Producing rat insulin using recombinant DNA, Walter Gilbert

Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.

  • ID: 15256
  • Source: DNAi

15087. Genentech's approach to making insulin, David Goeddel

David Goeddel was one of the first scientists Herb Boyer hired for the insulin project. Here, David Goeddel talks about Genentech's general strategy for synthesizing insulin.

  • ID: 15087
  • Source: DNAi