Website Search
ID 15644

Vats used to grow bacteria

Description:
How was synthetic insulin first made? Although the idea was simple, in practice there were substantial problems. No one had the human DNA sequence for insulin, and there was a moratorium on the production of human recombinant DNA. Two different strategies were used to obtain the human insulin DNA sequence, and transfer it into bacteria. In the end, one strategy and one company won out.
Transcript:
Vats used to grow bacteria. <br><br> How was synthetic insulin first made? Although the idea was simple, in practice there were substantial problems. No one had the human DNA sequence for insulin, and there was a moratorium on the production of human recombinant DNA.<br><br> Two different strategies were used to obtain the human insulin DNA sequence, and transfer it into bacteria. In the end, one strategy and one company won out.
Keywords:
human dna sequence,human insulin,recombinant dna,substantial problems,moratorium,bacteria
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Related content:

15505. Synthesizing human insulin using recombinant DNA, 3D animation with no audio
Synthesizing human insulin using recombinant DNA, 3D animation with no audio
15643. Genentech
Genentech, the first biotechnology company, established in 1976.
15928. How insulin is made using bacteria
Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels. Synthetic insulin i
15255. Producing human insulin using recombinant DNA, Walter Gilbert
Walter Gilbert talks about the reasons for making insulin with recombinant DNA.
15258. Government restrictions on working with recombinant DNA, Walter Gilbert
In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.
15929. How insulin is made using yeast
Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels. Synthetic insulin i
15259. Cloning the human insulin gene, Walter Gilbert
The recombinant DNA moratorium meant Gilbert had to go to England's Porton Down facility to try and isolate human insulin. He only had one chance ...
15927. Isolating DNA to make human insulin
Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.
15256. Producing rat insulin using recombinant DNA, Walter Gilbert
Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.
15087. Genentech's approach to making insulin, David Goeddel
David Goeddel was one of the first scientists Herb Boyer hired for the insulin project. Here, David Goeddel talks about Genentech's general strategy for synthesizing insulin.
Cold Spring Harbor Laboratory
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving