Making GeneChipsÂ® at Affymetrix :: CSHL DNA Learning Center

Making GeneChipsÂ® at Affymetrix

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A technician uses a computer guiding system to align the filter mask in perfect register with the quartz wafer. The filter mask will block light to selected regions of the wafer. More nucleotides can be added to the exposed regions.

quartz wafer,filter mask

ID: 15664
Source: DNALC.DNAi

15666. Making GeneChips® at Affymetrix

The quartz wafer is in the holding position on the DNA synthesizer. The wafer is moved to a vertical reaction vessel for the process of DNA chain elongation.

ID: 15666
Source: DNALC.DNAi

15662. Making GeneChips at Affymetrix

Here you can see a technician shuttling a quartz wafer (containing 50-400 GeneChips) between alternating photolithography and DNA synthesis steps.

ID: 15662
Source: DNAi

15665. Making GeneChips at Affymetrix

A technician loads a quartz wafer into the DNA synthesizer, where nucleotides will be added to selected sequences to build probes.

ID: 15665
Source: DNAi

15667. Making GeneChips® at Affymetrix

Here a technician aligns a quartz wafer, from which 50-400 GeneChips are prepared for mounting.

ID: 15667
Source: DNALC.DNAi

15663. Making GeneChips at Affymetrix

A technician prepares to load a filter mask into the photolithography camera. These filters allow the technician to build customized chains of DNA. The blue boxes on the right store 50-100 filters needed to produce a single type of GeneChip.

ID: 15663
Source: DNAi

15661. Making GeneChips at Affymetrix

Affymetrix, the makers of GeneChip arrays.

ID: 15661
Source: DNAi

15258. Government restrictions on working with recombinant DNA, Walter Gilbert

In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.

ID: 15258
Source: DNAi