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ID 15665
Making GeneChips™ at Affymetrix
Description:
A technician loads a quartz wafer into the DNA synthesizer, where nucleotides will be added to selected sequences to build probes.
Keywords:
quartz wafer,dna synthesizer,probes,sequences
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 15666. Making GeneChips® at Affymetrix
- The quartz wafer is in the holding position on the DNA synthesizer. The wafer is moved to a vertical reaction vessel for the process of DNA chain elongation.
- SOURCE: DNAi
- 15661. Making GeneChips™ at Affymetrix
- Affymetrix, the makers of GeneChip™ arrays.
- SOURCE: DNAi
- 15662. Making GeneChips™ at Affymetrix
- Here you can see a technician shuttling a quartz wafer (containing 50-400 GeneChips™) between alternating photolithography and DNA synthesis steps.
- SOURCE: DNAi
- 15664. Making GeneChips™ at Affymetrix
- A technician uses a computer guiding system to align the filter mask in perfect register with the quartz wafer. The filter mask will block light to selected regions of the wafer. More nucleotides can be added to the exposed regions.
- SOURCE: DNAi
- 15667. Making GeneChips® at Affymetrix
- Here a technician aligns a quartz wafer, from which 50-400 GeneChips are prepared for mounting.
- SOURCE: DNAi
- 15505. Synthesizing human insulin using recombinant DNA, 3D animation with no audio
- Synthesizing human insulin using recombinant DNA, 3D animation with no audio
- SOURCE: DNAi
- 15920. DNA arrays
- In the 1990s, DNA arrays provided the means to analyze patterns of gene expression at different timepoints in a living cell.
- SOURCE: DNAi
- 15922. Early DNA sequencing
- Two sequencing techniques were developed independently in the 1970s. The method developed by Fred Sanger used chemically altered "dideoxy" bases to terminate newly synthesized DNA fragments at specific bases (either A, C, T, or G). These fragments are th
- SOURCE: DNAi
- 15583. DNA fingerprint (single locus probe)
- DNA profiling, DNA fingerprinting.
- SOURCE: DNAi
- 15983. Today's DNA profile
- DNA fingerprints are also known as DNA profiles. They are now made using probes that detect differences in the number of short tandem repeats (STRs) between individuals.
- SOURCE: DNAi
