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ID 15918
Transformation
Description:
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is widely used today to obtain high-efficiency transforming cells.
Keywords:
dna transformation,calcium chloride,high efficiency,higa,rare event,akiko,e coli,mandel,bacteria,cells
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 16705. Animation 34: Genes can be moved between species.
- Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
- SOURCE: DNAFTB
- 16715. Video 34: Douglas Hanahan, clip 1
- Improving on the Mandel and Higa method of DNA transformation.
- SOURCE: DNAFTB
- 15916. DNA transformation
- Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
- SOURCE: DNAi
- 16395. Animation18: Bacteria and viruses have DNA too.
- Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
- SOURCE: DNAFTB
- 15162. Preparing the DNA for sequencing, Shane Yeager
- Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.
- SOURCE: DNAi
- 16687. Concept 33: Genes can be turned on and off.
- Organisms can regulate gene expression.
- SOURCE: DNAFTB
- 16657. Animation 31: Some DNA does not encode protein.
- Roy Britten presents his work with David Kohne on repetitive DNA and its evolutionary origins.
- SOURCE: DNAFTB
- 15638. Bacterium containing plasmids with foreign DNA
- Illustration of bacterium containing plasmids with foreign DNA.
- SOURCE: DNAi
- 16720. Biography 34: Stan Norman Cohen (1935 - )
- Stan Cohen and Herb Boyer "invented" recombinant DNA technology.
- SOURCE: DNAFTB
- 16613. Animation 28: Some types of mutations are automatically repaired.
- Stan Rupert explains UV damage and light-activated DNA repair systems and Richard Setlow's work on thymine dimer repair.
- SOURCE: DNAFTB
