Close but no cigar, Walter Gilbert

Interviewee: Walter Gilbert. Walter Gilbert talks about close but no cigar.

We had this, tried to obtain the human sequence, not the rat sequence, and tried to make it in large enough amounts to be commercially useful. In our case, we failed by a little bit, we had to make twenty milligrams per litre of insulin in order to become commercially useful, we got to nineteen, just under the cut-off line, and the project never became successful. Genentech's case they actually made the human insulin and that human insulin made by a somewhat different procedure, finally went to market. Nowadays of course the only insulin you would get on the market would be synthetically made by these recombinant DNA techniques.

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Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.

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15259. Cloning the human insulin gene, Walter Gilbert

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15505. Synthesizing human insulin using recombinant DNA, 3D animation with no audio

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16698. Video 33: Walter Gilbert, clip 5

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16697. Video 33: Walter Gilbert, clip 4

How Jacob and Monod showed the existence of the inhibitor (what Gilbert calls the repressor).

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