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ID 16715
Video 34: Douglas Hanahan, clip 1
Description:
Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.
Keywords:
douglas hanahan, dna transformation, dna uptake, transformation techniques, mandel, biochemistry mechanisms
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This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 16719. Video 34: Douglas Hanahan, clip 5
- Size of DNA and transformation efficiencies.
- SOURCE: DNAFTB
- 16716. Video 34: Douglas Hanahan, clip 2
- The problem of getting DNA into a bacteria.
- SOURCE: DNAFTB
- 16718. Video 34: Douglas Hanahan, clip 4
- Definitions of "cloning."
- SOURCE: DNAFTB
- 16717. Video 34: Douglas Hanahan, clip 3
- How big are those bacterial pores?
- SOURCE: DNAFTB
- 16722. Biography 34: Doug Hanahan (1951 - )
- Doug Hanahan refined transformation methods for DNA uptake into bacteria.
- SOURCE: DNAFTB
- 15918. Transformation
- DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
- SOURCE: DNAi
- 15916. DNA transformation
- Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
- SOURCE: DNAi
- 16705. Animation 34: Genes can be moved between species.
- Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
- SOURCE: DNAFTB
- 15012. Oswald Avery
- In 1944, Avery and MacLeod, and McCarty purified the transforming principle of Fred Griffith's earlier experiment with pneumocossus bacteria. By a process of elimination, these scientists showed that DNA was the factor that caused genetic transformation.
- SOURCE: DNAi
- 15074. Taking apart plasmid DNA, Stanley Cohen
- Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
- SOURCE: DNAi
