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ID 16715

Video 34: Douglas Hanahan, clip 1

Description:
Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.
Keywords:
douglas hanahan, dna transformation, dna uptake, transformation techniques, mandel, biochemistry mechanisms
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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16719. Video 34: Douglas Hanahan, clip 5
Size of DNA and transformation efficiencies.
16716. Video 34: Douglas Hanahan, clip 2
The problem of getting DNA into a bacteria.
16718. Video 34: Douglas Hanahan, clip 4
Definitions of "cloning."
16717. Video 34: Douglas Hanahan, clip 3
How big are those bacterial pores?
16722. Biography 34: Doug Hanahan (1951 - )
Doug Hanahan refined transformation methods for DNA uptake into bacteria.
15918. Transformation
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
15916. DNA transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
16705. Animation 34: Genes can be moved between species.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
15012. Oswald Avery
In 1944, Avery and MacLeod, and McCarty purified the transforming principle of Fred Griffith's earlier experiment with pneumocossus bacteria. By a process of elimination, these scientists showed that DNA was the factor that caused genetic transformation.
15074. Taking apart plasmid DNA, Stanley Cohen
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
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