Video 34: Douglas Hanahan, clip 5

Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.

mechanisms, biochemistry, ucsf, efficiencies, transformation techniques, douglas hanahan, dna uptake

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16715. Video 34: Douglas Hanahan, clip 1

Improving on the Mandel and Higa method of DNA transformation.

  • ID: 16715
  • Source: DNAFTB

16716. Video 34: Douglas Hanahan, clip 2

The problem of getting DNA into a bacteria.

  • ID: 16716
  • Source: DNAFTB

16717. Video 34: Douglas Hanahan, clip 3

How big are those bacterial pores?

  • ID: 16717
  • Source: DNAFTB

16718. Video 34: Douglas Hanahan, clip 4

Definitions of "cloning."

  • ID: 16718
  • Source: DNAFTB

16722. Biography 34: Doug Hanahan (1951 - )

Doug Hanahan refined transformation methods for DNA uptake into bacteria.

  • ID: 16722
  • Source: DNAFTB

15916. DNA transformation

Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

  • ID: 15916
  • Source: DNAi

15918. Transformation

DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is

  • ID: 15918
  • Source: DNAi

16705. Animation 34: Genes can be moved between species.

Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.

  • ID: 16705
  • Source: DNALC.DNAFTB

938. Hallmarks, Overview: Hanahan

Professor Douglas Hanahan discusses how cancer acquires capabilities and these capabilities are all, to some approximation, necessary to produce a successful tumor.

  • ID: 938
  • Source: IC

942. Hallmarks, Evading death: Hanahan

Professor Douglas Hanahan explains that a fundamental property of multi-cellular organisms is the capability to have cells commit suicide or undergo apoptosis, which is a form of programmed cell death.

  • ID: 942
  • Source: IC