|A collaboration of the New York City Department of Education and the Dolan DNA Learning Center, Cold Spring Harbor Laboratory
Genetics and Biotechnology Footlocker Kits
To assist in the implementation of targeted labs, Footlocker Kits are available for rental by eligible teachers (see below).
Prior participation in a Harlem DNA Lab Professional Development workshop(s) corresponding to the Footlocker Kit of choice is required to be eligible for kit rental. Each workshop session integrates the use of corresponding Footlocker Kits, so teachers are familiar with equipment use and lab preparation for each activity. Additionally, participants are introduced to the Lab Center, an online resource designed to provide teacher support in the form of lesson plans, student worksheets, lab protocols and biomedia links for each of the targeted labs. There are no workshops scheduled at this time.
If you would like to sign up to rent a kit, please download and complete the Footlocker Application Form and email it to Melissa Lee at firstname.lastname@example.org.
When you pick up your kit, you need to submit a Student Demographic Form for each class that will use the kit. These can also be emailed to Melissa Lee at email@example.com.
Available Footlocker Kits:
Variability, Inheritance, DNA Structure, and DNA Isolation
4 Labs: 1 CASE
This kit includes materials to complete four basic lab activities:
NOTE: Teacher is responsible for labeling tubes and aliquoting solutions for the DNA extraction. All pertinent worksheets, protocols and observation sheets can be printed from the Lab Center at: http://www.dnalc.org/labcenter/harlem/.
Bacterial Transformation or Protein Isolation
1 Lab, 2 CASES
Observe first-hand that genes code for traits! Using a plasmid vector, harmless E.coli bacteria are transformed with the GFP (Green Fluorescent Protein) gene, normally found in the Pacific jellyfish Aequoria Victoria. If successful, the bacteria will produce the bioluminescent jellyfish protein, and glow! This experiment utilizes techniques used by pharmaceutical companies to make human proteins such as Insulin. By special request only, an additional set of materials can be included to extract and purify GFP from transformed bacteria. Includes:
NOTE: Teacher is responsible for labeling tubes and aliquoting solutions for this lab, and must supply ice during lab and bleach for clean-up. Teacher must have a refrigerator to store reagents and Petri dishes. All pertinent worksheets, protocols and observation sheets can be printed from the Lab Center at: http://www.dnalc.org/labcenter/harlem/.
DNA Analysis and Forensics
1 Lab, 3 CASES
In this lab, students use the same techniques that scientists and detectives use to analyze DNA. DNA is digested by EcoRI, Hind III and "mystery" restriction enzymes. The resulting DNA fragments and their unique gel patterns or "fingerprints" are analyzed by gel electrophoresis, and the "mystery" enzyme is indentified. Includes:
NOTE: Teacher is responsible for labeling tubes and aliquoting solutions. Teacher must also supply a heat source (microwave) for melting agarose, a refrigerator/freezer for storage of reagents and bottled water in 1-liter bottles to make TBE electrophoresis buffer. All pertinent worksheets, protocols and observation sheets can be printed from the Lab Center at: http://www.dnalc.org/labcenter/harlem/.
PCR and Human DNA Variation, Parts I & II
1 Lab, 2 CASES
In this lab students will extract and amplify their own DNA.* Using Polymerase Chain Reaction (PCR) each participant will amplify a specific region of his/her own DNA and use gel electrophoresis to analyze the results. Teachers have the option of using primers to:
NOTE: Teacher is responsible for labeling tubes and aliquoting solutions. Teacher must also supply plastic or paper cups, bottled water and salt to make and aliquot saline solution, heat source (microwave) to melt agarose and refrigerator/freezer for storage of reagents. All pertinent worksheets, protocols and observation sheets can be printed from Lab Center.
* By law, any student under the age of 18 must submit a permission slip (included in kit) signed by a parent or guardian prior to participation in this lab.