Website Search
ID 15023

The experiment that galvanized the scientific community, Paul Berg

Interviewee: Paul Berg. Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA. (DNAi Location: Manipulation > Revolution > Players > Paul Berg > A jolt to the scientific community)
You could take a colony and put it into a hundred gallon vat and the bacteria would grow up and fill up the vat, and every cell in that vat would contain the piece of DNA that the original bacterium picked up when you mixed them with the DNA. So that showed you could clone DNA, and I think that experiment is what galvanized the scientific community. It is in fact the experiment that motivated the moratorium letter, because it became clear you could put any kind of DNA into that plasmid and get it into a bacterium, and so you could put toxin genes, you could put drug-resistant genes, any kind of DNA you had access to could be put into a plasmid, put into a bacterium and cloned.
recombinant dna,paul berg,rdna,dnai,bacterium,interviewee,moratorium,firestorm,jolt,toxin,genes,bacteria,vat,manipulation,controversy
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Related content:

15021. The moratorium letter regarding risky experiments, Paul Berg
In 1974, scientists in the field of recombinant DNA drafted a letter calling upon "scientists throughout the world" to suspend certain types of studies until hazards could be assessed. Paul Berg talks about the "Moratorium Letter."
15024. The origin and utility of recombinant DNA, Paul Berg
Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
15020. Possible dangers of recombinant DNA, Paul Berg
Paul Berg talks about possible dangers of recombinant DNA.
15158. Bridging evolutionary barriers, Robert Pollack
Renowned biologist and philosopher Robert Pollack reflects on his concern over the potential danger of Janet Mertz's experiment inserting a cancer-causing gene from a monkey virus into a bacterium that lives in humans.
15018. Outrage over recombinant DNA, Paul Berg
Paul Berg's student, Janet Mertz, planned an experiment that would recombine DNA from a monkey virus with DNA from a bacterium that lives in the human gut. Berg describes colleague Bob Pollack's outrage at this.
15025. On the phenomenon of restriction, Paul Berg
Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA.
15133. Why Asilomar conference was a bad idea, Victor McElheny
Former New York Times science journalist Victor McElheny talks about why he thought the "Moratorium Letter" was asking for trouble.
15022. How the first recombinant DNA was created, Paul Berg
Paul Berg speaks about his student Janet Mertz's experiment to make the first recombinant DNA molecule.
15017. Reaction to outrage over recombinant DNA, Paul Berg
Paul Berg recollects his reaction to his colleague Bob Pollack's opposition to experimentation with recombinant DNA.
15653. Asilomar meeting
Asilomar meeting. February 1975. (L to R) Maxine Singer, Norton Zinder, Sydney Brenner, Paul Berg.
Cold Spring Harbor Laboratory
CSHL HomeAbout CSHLResearchEducationPublic EventsNewsstandPartner With UsGiving