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ID 15024

The origin and utility of recombinant DNA, Paul Berg

Interviewee: Paul Berg. Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes. (DNAi Location: Manipulation > Revolution > Players > Paul Berg > Why recombinant DNA is useful)
And that is the critical step, the recognition that you could take a complex mixture of a million different pieces of DNA and separate them physically one from another by putting them into a bacterial cell and allow the bacteria to amplify them. That's the origin of recombinant DNA, the real utility of recombinant DNA was the ability to cut pieces of DNA up with restriction enzymes, join them to a plasmid, put them into a bacterium and then separate the bacteria so you had different pieces of DNA.
restriction enzymes,recombinant dna,paul berg,dnai,bacterial cell,critical step,interviewee,bacterium,bacteria,genes,manipulation,mixture
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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