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ID 15264

Using argobacterium to genetically engineer plants, Robert Horsch

Interviewee: Robert Horsch. Robert Horsch explains the mechanism by which agrobacterium delivers its DNA "parcel." (DNAi Location: Manipulation > Techniques > Transferring & storing > Interviews > Using agrobacterium)
As it turns out the agrobacterium doesn't know or care what's in the package of DNA that it transfers into the plant cell genome. And so we can go in with recombinant techniques and cut out the genes that make the gall, cut out the genes that make the opines, and put in any genes that you can engineer. Then the bacterium will transfer it without knowing any difference, and we therefore have a way to engineer a living plant cell using this natural system.
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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