Website Search
ID 15527

DNA microarray

DNA microarray
The grid you can see in this microarray slide is actually composed of 30,000 individual DNA dots, each targeted to match a specific human gene. In order to work out which genes are being expressed, messenger RNA is first extracted from the cell sample and copied back to DNA using an enzyme. This DNA, called cDNA, is complementary to the target gene, so we'll associate with it, or hybridize with it, on the slide. After labeling with a fluorescent dye, the cDNA is washed over the slide. The genes currently active in the cell can then be identified by the level of the fluorescence and the color of the spots.
dna microarray,target gene,messenger rna,gene analysis,human gene,genes,fluorescent dye,dots,large scale,animation
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Related content:

15542. DNA microarray output
DNA microarrays provide the means to analyze patterns of gene expression at different timepoints in a living cell.
16529. Animation 24: The RNA message is sometimes edited.
Rich Roberts and Phil Sharp explain restriction enzymes, electrophoresis, and split genes.
553. Whole Genome Association
Special techniques are used for screening each individual’s genome for millions of different SNPs. This kind of comparison is referred to as a genome-wide association study.
15992. DNA microarrays
DNA microarrays provide the means to analyze patterns of gene expression at different timepoints in a living cell.
16736. Animation 36: Different genes are active in different kinds of cells.
Igor Dawid and Thomas Sargent explain how they developed subtractive mRNA hybrization to find genes expressed by different cell types. Pat Brown and Steve Fodor show how genomes can be screened with DNA arrays and GeneChips™
793. Whole Genome Association
Jonathan Sebat, a researcher at Cold Spring Harbor Laboratory, discusses how association studies are used to determine the causes of genetic disorders.
15670. Making GeneChips® at Affymetrix
The last step! A technician applies the label and serial number to a GeneChip cassette. The GeneChip is now ready to be used in a laboratory to perform large-scale genetic analysis.
1438. Gene Expression
Doctor Josh Dubnau explains that some genes are preferentially active in one part of the brain or body, while other genes are particular active in another location.
15036. Why we developed the microarray, Patrick Brown
Pat Brown talks about developing microarray technology for genome-wide analysis.
1262. Microarray Studies of Schizophrenia
Professor David Lewis outlines how microarrays have transformed the search for schizophrenia genes and led to his group's discovery of the candidate gene, RGS4.
Cold Spring Harbor Laboratory
CSHL HomeAbout CSHLResearchEducationPublic EventsNewsstandPartner With UsGiving