Making GeneChips® at Affymetrix

The quartz wafer is in the holding position on the DNA synthesizer. The wafer is moved to a vertical reaction vessel for the process of DNA chain elongation.

The quartz wafer is in the holding position on the DNA synthesizer. The wafer is moved to a vertical reaction vessel for the process of DNA chain elongation.

quartz wafer,dna synthesizer,dna chain,reaction vessel,elongation

  • ID: 15666
  • Source: DNALC.DNAi

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15665. Making GeneChips™ at Affymetrix

A technician loads a quartz wafer into the DNA synthesizer, where nucleotides will be added to selected sequences to build probes.

  • ID: 15665
  • Source: DNAi

15662. Making GeneChips™ at Affymetrix

Here you can see a technician shuttling a quartz wafer (containing 50-400 GeneChips™) between alternating photolithography and DNA synthesis steps.

  • ID: 15662
  • Source: DNAi

16515. Animation 23: A gene is a discrete sequence of DNA nucleotides.

Fred Sanger outlines DNA sequencing.

  • ID: 16515
  • Source: DNALC.DNAFTB

15625. Polymerase chain reaction (PCR)

DNA polymerase (blue) makes many copies of DNA (red) in a cycle of the polymerase chain reaction (PCR).

  • ID: 15625
  • Source: DNAi

17044. Polymerase chain reaction (PCR)

Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA.

  • ID: 17044
  • Source: DNALC

15661. Making GeneChips™ at Affymetrix

Affymetrix, the makers of GeneChip™ arrays.

  • ID: 15661
  • Source: DNAi

15624. Kary Mullis

Image of Kary Mullis. In 1985, Kary Mullis invented the polymerase chain reaction (PCR), a method of amplifying or producing many copies of a specific piece of DNA. The revelation came to this eccentric character on a drive in northern California.

  • ID: 15624
  • Source: DNAi

15138. Naming PCR

Kary Mullis explains how the polymerase chain reaction (PCR) was named.

  • ID: 15138
  • Source: DNAi

15664. Making GeneChips® at Affymetrix

A technician uses a computer guiding system to align the filter mask in perfect register with the quartz wafer. The filter mask will block light to selected regions of the wafer. More nucleotides can be added to the exposed regions.

  • ID: 15664
  • Source: DNALC.DNAi

15475. The cycles of the polymerase chain reaction (PCR), 3D animation

The cycles of the polymerase chain reaction (PCR).

  • ID: 15475
  • Source: DNALC.DNAi