Interviewee: Robert Horsch.
Robert Horsch talks about the parasitic nature of agrobacterium and the effect it has on the host plant.
(DNAi Location: Manipulation > Techniques > Transferring & storing > Interviews > What is agrobacterium?)
Transcript:
Agrobacterium is amazing little bacteria that is found in soil almost everywhere in the world and that makes its living in nature by genetically engineering live cells in a wounded part of a plant. It will splash in or blow in on dust particles, and when it finds a wound it will attach to the cell wall and transfer a segment of DNA that contains genes that do two things: the first are a set of genes that cause the plant cells to grow into this gall, and that creates a physical home, it's full of little nooks and crannies that the bacterial colonies can grow and protect it from the weather. It also tells the plant cells to make a unique set of compounds called opines that are nitrogen-rich, which is an essential rare nutrient in nature, and have sugar, an energy and carbon source.
Keywords:
recombinant dna technology,robert horsch,nooks and crannies,what is agrobacterium,bacterial colonies,manipulation techniques,plant cells,dust particles,dnai,carbon source,host plant,opines,interviewee,weather,genes,nitrogen,compounds,bacteria,segment,soil
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.