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ID 15537

Shotgun sequencing and dealing with repeat sections, 3D animation with basic narration

Description:
The private project blew the genome apart into pieces of different sizes and used mathematical models and other maps to reconstruct the genome from these pieces. (DNAi Location: Genome > The Project > Putting it together > Animations > Whole genome shotgun-private)
Transcript:
Shotgun sequencing is the method that was used by the private genome project. Shotgun sequencing requires multiple copies of the genome, which are effectively blown up into millions of small fragments. Each fragment is then sequenced. The small fragments are assembled using an immense amount of computer power to match overlapping sections. The drawback of this method comes when dealing with repeat sequences. Often there is no way of knowing how long the repeat sequence is; or in which of many different possible positions the fragments overlap. Even the incredibly powerful software used to shotgun sequence the human genome couldn't cope with this. So Celera, the private company which relied on this approach, had to use the public data to fill in the gaps left by the repeats.
Keywords:
shotgun sequencing,powerful software,computer power,genome project,human genome,fragments,drawback,narration,fragment,private company,gaps,sequences,animation
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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