Taking apart plasmid DNA, Stanley Cohen

Interviewee: Stanley Cohen. Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA. (DNAi Location: Manipulation > Techniques > Cutting and pasting > Rearranging plasmid DNA)

So the question came up, could we, while the plasmid DNA was outside of a cell, could we take the plasmid apart, rearrange it in different ways or add genes to an additional plasmid, and then use the transformation procedure to put the composite plasmid, we didn't use the term recombinant plasmid, back into cells.

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15646. Herbert Boyer

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