Interviewee: Stanley Cohen.
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
(DNAi Location: Manipulation > Techniques > Cutting and pasting > Rearranging plasmid DNA)
So the question came up, could we, while the plasmid DNA was outside of a cell, could we take the plasmid apart, rearrange it in different ways or add genes to an additional plasmid, and then use the transformation procedure to put the composite plasmid, we didn't use the term recombinant plasmid, back into cells.
herbert boyer,stanley cohen,manipulation techniques,recombinant dna,recombinant plasmid,dnai,interviewee,plasmid dna,genes,different ways,cells,transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Herbert Boyer: Former varsity lineman turned biotech bigwig. Expert at cutting DNA before most people knew it could be done.
Stanley Cohen: A born tinkerer; figured out the trick of using loops of DNA called plasmids to transform bacterial DNA
Image of Herbert Boyer. From lineman on the varsity football team to co-founder of the first biotech company, Boyer has never lacked imagination, drive, or vision. His and Stanley Cohen's recombinant DNA work paved the way for the biotech revolution.