Website Search
ID 15349
RNA can stimulate protein synthesis, Marshall Nirenberg
Description:
Interviewee: Marshall Nirenberg.
RNA can stimulate protein synthesis.
Transcript:
Everything, everything fell into place, everything worked. I mean we soon found that adding RNA, which would have been prepared from ribosomes, to cell-free extracts of E. coli stimulated amino acid incorporation into protein. All the controls were there, even though the stimulation was tiny, it was like, like thirty counts per minute above a background of maybe a hundred or eighty counts per minute. It was a small stimulation but it was real, and when I saw that, I mean the very first experiment, I knew it was true, I knew that RNA was stimulating, that template RNA was stimulating cell-free protein synthesis, I jumped for joy because we had really, we had really found that RNA, messenger RNA could stimulate protein synthesis.
Keywords:
marshall nirenberg,cell free protein synthesis,protein amino acids,free extracts,messenger rna,interviewee,e coli,ribosomes,incorporation
Downloads:
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 15347. Cell-free protein synthesis, Marshall Nirenberg
- Marshall Nirenberg talks about cell-free protein synthesis.
- SOURCE: DNAi
- 15882. Breaking the code
- Marshall Nirenberg and Heinrich Matthaei used poly-U mRNA in a cell-free system to make a polyphenylalanine protein chain. This showed that UUU must be the code that specifies the amino acid phenylalanine.
- SOURCE: DNAi
- 15885. Cell-free extracts
- Paul Zamecnik first developed the cell-free extract system, which Marshall Nirenberg adapted to decipher the genetic code. Paul Zamecnik and Mahlon Hoagland also isolated activated tRNA, the "adaptor" that shuttled amino acids to ribsomes for incorporati
- SOURCE: DNAi
- 16489. Biography 21: Paul Charles Zamecnik (1913-)
- Paul Zamecnik developed a cell-free extract that he and Mahlon Hoagland used to study protein synthesis. They identified tRNA as the adaptor predicted by Francis Crick in his Central Dogma
- SOURCE: DNAFTB
- 16494. Animation 22: DNA words are three letters long.
- Several researchers crack the genetic code.
- SOURCE: DNAFTB
- 16471. Animation 21: RNA is an intermediary between DNA and protein.
- Francis Crick describes RNA and its role and Paul Zamecnick explains protein synthesis.
- SOURCE: DNAFTB
- 15350. The RNA code for phenylalanine, Marshall Nirenberg
- Marshall Nirenberg talks about the RNA code for phenylalanine.
- SOURCE: DNAi
- 15353. Figuring out the other codons, Marshall Nirenberg
- After decoding the "easy" codons, Marshall Nirenberg talks about his strategy for decoding the rest.
- SOURCE: DNAi
- 15001. Paul Zamecnik
- Paul Zamecnik photo
- SOURCE: DNAi
- 1023. Pathways, Making the protein
- In this section learn that in the cytoplasm, the messenger RNA is released from its carrier proteins and binds to a protein assembly complex called a ribosome.
- SOURCE: IC
