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ID 15927
Isolating DNA to make human insulin
Description:
Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.
Keywords:
isolating dna,rat insulin,human insulin,dna sequence,walter gilbert,gilbert walter
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 15256. Producing rat insulin using recombinant DNA, Walter Gilbert
- Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.
- SOURCE: DNAi
- 16117. Close but no cigar, Walter Gilbert
- Walter Gilbert talks about close but no cigar.
- SOURCE: DNAi
- 15259. Cloning the human insulin gene, Walter Gilbert
- The recombinant DNA moratorium meant Gilbert had to go to England's Porton Down facility to try and isolate human insulin. He only had one chance ...
- SOURCE: DNAi
- 15088. Gilbert's approach to synthesizing insulin, David Goeddel
- David Goeddel talks about how another group was trying to make insulin.
- SOURCE: DNAi
- 15255. Producing human insulin using recombinant DNA, Walter Gilbert
- Walter Gilbert talks about the reasons for making insulin with recombinant DNA.
- SOURCE: DNAi
- 15258. Government restrictions on working with recombinant DNA, Walter Gilbert
- In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.
- SOURCE: DNAi
- 15257. Trying to produce insulin using recombinant DNA, Walter Gilbert
- Walter Gilbert talks about his feelings regarding the insulin project and science in general.
- SOURCE: DNAi
- 16697. Video 33: Walter Gilbert, clip 4
- How Jacob and Monod showed the existence of the inhibitor (what Gilbert calls the repressor).
- SOURCE: DNAFTB
- 16698. Video 33: Walter Gilbert, clip 5
- Jacob and Monod never identified the inhibitor, but Gilbert found it.
- SOURCE: DNAFTB
- 16694. Video 33: Walter Gilbert, clip 1
- Before Jacob and Monod, people thought the amount of protein in a cell was constant and proteins turned themselves off.
- SOURCE: DNAFTB
