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ID 16118

Recombinant DNA, David Goeddel

Description:
Interviewee: David Goeddel. David Goeddel talks about recombinant DNA.
Transcript:
So taking the synthetic approach, where you synthesize a, a fragment of DNA, let's say coding insulin, now you have this linear piece of DNA, but it\'s not in any form that it can do anything you need to put it in a replicating form. And the most common way to do that is insert it into what's called a bacterial plasmid, which is a small circular piece of DNA that are found in some bacteria. And this can be opened up, essentially with molecular scissors, that allow your synthetic piece to be inserted there and all of it sealed back up, essentially glued back together. Now this circular piece of DNA can easily be put back into the bacteria, where now it is replicated, over and over, in exact copies. And that\'s the recombinant piece, you\'ve taken your piece of DNA and re-combined it into another piece of DNA that can now be replicated in an organism.
Keywords:
bacterial plasmid,molecular scissors,recombinant dna,circular piece,synthetic approach,exact copies,fragment,organism,insulin,bacteria
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Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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