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ID 15022

How the first recombinant DNA was created, Paul Berg

Interviewee: Paul Berg. Paul Berg speaks about his student Janet Mertz's experiment to make the first recombinant DNA molecule. (DNAi Location: Manipulation > Techniques > cutting & pasting > Interviews > First recombinant DNA)
The first recombinant DNA made by using enzyme-created sticky ends, cohesion ends, cohesive ends, was actually done by my student, Janet Mertz. She took two different DNAs, each of them had distinguishable properties, mixed them or, or cut them with the enzyme, mixed them, added an enzyme that could join ends to ends, and then showed she had created a molecule that now shared the properties of the two starting materials.
dna recombination,dna molecule,restriction enzymes,recombinant dna,manipulation techniques,paul berg,sticky ends,dnai,mertz,dnas,interviewee,cohesion
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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15019. Cohesive (sticky) ends and their significance in genetic engineering, Paul Berg
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15421. Cutting DNA with restriction enzymes, James Watson
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